Tremella fuciformis?polysaccharide?(TFP) is an important bioactive substance in?T. fuciformis, which contributes to its application as medicine and food. In the present work, TFP was extracted with enzymatic combined with wet beating and its physicochemical as well as biological activities were investigated in the study. The result showed that the purity of TFP1 reached 92.14% (m/m). The TFP1, purified by?DEAE?column, had a molecular weight (Mw) of 5.8 × 103?kDa and consisted mainly of?mannose,?xylose,?fucose, glucose and?glucuronic acid?at a molar ratio of 1.91: 0.1: 2.49: 6.23: 0.95. The structure of TFP1 was preliminarily investigated by?infrared spectroscopy, nuclear magnetic resonance and?atomic force microscope?(AFM) analysis. Moreover, the?antioxidant assays?showed that TFP1 could scavenge DPPH,?ABTS+?and?hydroxyl radicals. The excellent?water holding capacity?of TFP1 implied its application in the food, pharmaceutical andcosmetic industries.
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