Reduced immunity can harm the health of the organism, and nowadays, improving immunity is getting more and more attention, so the nutrients with immune boosting function (acerola cherry, taurine, zinc gluconate, and lactoferrin) are compounded in the best ratio to develop a nutritional formula food, and evaluated by cellular immunity, humoral immunity, non-specific immunity. In this study, an immunocompromised mice model was established using cyclophosphamide (CTX), the ability and difference of different components to enhance the immunity of mice were determined by the gavage of different components. The results showed that the nutritional formula food could recover the body weight of immunocompromised mice, promote the development of immune organs in immunocompromised mice, enhance the delayed-type hypersensitivity (DTH) response, the ability to produce serum hemolysin and the phagocytosis of monocytes in immunocompromised mice, and increase the levels of immunoglobulin A (IgA), IgG and IgM in the serum of immunocompromised mice. It has proved that this nutritional formula food (containing acerola cherry, taurine, zinc gluconate, and lactoferrin) has synergistic effect and can work together on humoral immunity, cellular immunity and non-specific immunity to improve the immune resistance of mice, and has promising application.