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—— 中國食品雜志社
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磁微粒酶聯(lián)免疫吸附法測定玉米中的伏馬毒素B1
來源：食品科學網(wǎng) 閱讀量: 175 發(fā)表時間: 2017-06-07
作者: 管 笛，潘燦平，王 文，武會娟
關鍵詞: magnetic particles; enzyme-linked immunosorbent assay; fumonisins; corn; protein A
摘要:

Magnetic particles (MPs) were used as the support to replace enzyme-linked immunosorbent assay (ELISA) plates for
the determination of fumonisin B1 (FB1) in corn. Goat anti-rabbit antibody and protein A-MP conjugates were utilized in a direct
competitive ELISA format. Under optimized conditions, the limits of detection (LOD) for goat anti-rabbit antibody and protein
A-MPs were 0.024 and 0.030 μg/mL, respectively. The goat anti-rabbit antibody MP conjugates exhibited a lower LOD which
was only one third of that of conventional ELISA. The recoveries from spiked corn varied from 76.4% to 110.6%, with relative
standard deviation (RSD) less than 11%. Overall, a sensitive and stable method for FB1 has been developed in this paper.

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